Step1: Get Subtotal with currency Symbol:
<?php
$totals = Mage::getSingleton('checkout/session')->getQuote()->getTotals(); //Total object
$grandtotal = round($totals["grand_total"]->getValue()); //Grandtotal value
echo $formattedPrice = Mage::helper('core')->currency($grandtotal , true, false);
?>
Step2: Get Subtotal without currency Symbol:
<?php echo Mage::helper('checkout/cart')->getQuote()->getSubtotal() ?>